Identification of Cryptosporidium oocyst and Giardia cyst in of the samples of raw surface water of Kan River in Tehran Giardia cyst in the water of Kan River in Tehran

Main Article Content

Milad Mahdavi
Abdolhossein Dalimi
Fatemeh Ghaffari far


Background: Giardia and Cryptosporidium are considered as the most important causual agents of non-bloody diarrhea especially among primary school children in many countries including Iran. Many rivers are contaminated with Cryptosporidium oocyst and Giardia lamblia cysts due to domestic wastewater or farms wastewater and also the living of rodents on their margins. The present study aims to evaluate Kan River contamination with Cryptosporidium oocyst and Giardia cyst in Tehran by molecular method.

Method: sampling was conducted from different parts of Kan River in different seasons in 2019. Firstly, the smear has been prepared from sediments after filtering the water and collecting the sediment, and stained with trichrome and acid-fast methods and finally were examined microscopically. Then, they were amplified with Nested-PCR method by using the specific primers, the giardian gene from Giardia and 18s rRNA gene from Cryptosporidium. Positive samples were sequenced and phylogenetic tree was drawn.

Results: After the microscopic investigation, 12 suspected samples of Giardia cyst and 2 suspected samples of Cryptosporidium oocyst were detected, but only 4 samples infected with Giardia lamblia were molecularly found and no Cryptosporidium infection was observed. In terms of genotype, the identified Giardia was 100% consistent with human isolates of genotype B.

Conclusion: The presence of Giardia lamblia cyst in the water of Kan River indicates the contamination of this river to human contaminating parasites.

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How to Cite
Mahdavi, M., Dalimi, A., & Ghaffari far , F. (2024). Identification of Cryptosporidium oocyst and Giardia cyst in of the samples of raw surface water of Kan River in Tehran: Giardia cyst in the water of Kan River in Tehran. Afghanistan Journal of Infectious Diseases, 2(2), 1–8.
Research Article


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