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Main Manuscript.doc
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AJID SA Dr Daimi 103- RTL 7 JUn 2025.doc
AJID SA Dr Daimi 103- RTL 7 JUn 2025.doc
AJID SA Dr Daimi 103- RTL 7 JUn 2025.doc
10- AJID SA Dr Dalimi 103- RTG 7 Jun 2025 (2).pdf
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10.60141/ajid.103
Rapid Diagnosis of Acanthamoeba Keratitis by Iodine Staining of Corneal Scrapings
<p><strong>Background:</strong> Early and accurate diagnosis of <em>Acanthamoeba keratitis</em> is crucial for appropriate clinical management, preventing permanent corneal damage, scarring, or even perforation. We aimed to introduce a rapid diagnostic method for <em>A. keratitis</em>.</p>
<p><strong>Methods</strong>: Corneal scrapings from 21 patients with culture- and PCR-confirmed <em>A. keratitis</em> were evaluated using Giemsa and iodine staining. All cases were referred to Farabi Eye Hospital, a tertiary care center in Tehran, Iran, for specialized evaluation and management.</p>
<p><strong>Results</strong>: <em>Acanthamoeba</em> was detected in 42.33% of samples stained with Giemsa and 100% of samples stained with iodine.</p>
<p><strong>Conclusion</strong>: Iodine staining is a simple, cost-effective, and rapid method for diagnosing <em>A. keratitis</em>.</p>
https://creativecommons.org/licenses/by/4.0
Abdolhossein Dalimi, Fariba Khoshzaban, Mahmoud Jabbarvand
2025
iodine staining
diagnosis
Acanthamoeba keratitis
Abdolhossein
Dalimi
Department of Parasitology, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
IR
dalimi_a@modares.ac.ir
Fariba
Khoshzaban
Department of Parasitology, School of Medicine, Shahed University of Medical Sciences, Tehran, Iran
IR
fkhosh_99@yahoo.com
Mahmoud
Jabbarvand
Department of Ophthalmology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
IR
fkhosh_99@yahoo.com
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Third Volume, second Issue, July 2025
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